Development of Higher Order Thinking Skills (HOTS) Assessment in Plant and Animal Reproduction Systems

Authors

  • Erna Juwita Pakuan University
  • Prasetyorini Prasetyorini Pakuan University
  • Dadang Jaenudin Pakuan University
  • Vinsensius Felix Putra Andy Liman University of the People

Keywords:

Higher Order Thinking Skills, HOTS Assessment, 4D Model, Science Education, Reproduction System, Item Development

Abstract

Background: Assessment practices in schools are still largely dominated by Lower Order Thinking Skills (LOTS) items, with 72% categorized as C1 and the remainder as C2, limiting students’ opportunities to develop higher-order thinking skills such as analysis, evaluation, and creation. Therefore, an appropriate assessment design strategy is needed to support the development of Higher Order Thinking Skills (HOTS), particularly in science learning on the topic of plant and animal reproduction.

Methods: This study employed a research and development approach using the Four-D (4D) model, which consists of Define, Design, Develop, and Disseminate stages. Data were collected through analysis of existing assessment practices, expert validation, empirical testing, and responses from students and teachers.

Results: The Design stage produced 30 HOTS items aligned with core competencies and learning indicators and supported by contextual stimuli. The Develop stage indicated that the instrument was highly feasible, with expert validation results averaging 90.63% (Aiken’s V = 0.875). Empirical testing showed that 24 items were valid, with a reliability coefficient of 0.868, moderate difficulty levels, and good discrimination indices. Statistical analysis revealed significant differences in scores based on gender (p = 0.000), where female students performed better at C4–C5 levels, while male students excelled at C6. Student responses were highly positive (98.70%), and teachers confirmed the effectiveness of the assessment in measuring higher-order thinking skills.

Conclusion: The HOTS assessment design strategy based on the 4D model is effective in producing valid, reliable, and pedagogically relevant instruments, and it contributes to improving the quality of science education, particularly in fostering students’ higher-order thinking skills.

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Published

12-05-2026

How to Cite

Juwita, E., Prasetyorini, P., Jaenudin, D., & Felix Putra Andy Liman, V. (2026). Development of Higher Order Thinking Skills (HOTS) Assessment in Plant and Animal Reproduction Systems. JSEP (Journal of Science Education and Practice), 10(1). Retrieved from https://jsep-pasca.unpak.ac.id/index.php/jsep/article/view/22