Genetic Engineering Misconceptions in High School Biology Textbooks
DOI:
https://doi.org/10.33751/jsep.v10i1.15Keywords:
biology textbook, genetic engineering, misconceptionAbstract
Background: In 12th-grade high school biology classes, genetic engineering is a complex subject that is prone to misconceptions. The molecular processes involved, along with the lack of practical experiments, are the primary factors contributing to this. Meanwhile, textbooks are the primary teaching materials used by teachers to instruct students on genetic engineering in class. This study aimed to analyze the existence of misconceptions about genetic engineering in 12th-grade high school biology textbooks in Yogyakarta, particularly in Sleman Regency.
Methods: This study was a qualitative content analysis. The research subjects consisted of 12th-grade high school biology textbooks. The textbooks were selected using purposive sampling, which involved choosing the 2 books most widely used by teachers in schools, based on the results of interviews. Data collection was conducted through content analysis using a misconception analysis sheet as an instrument. Data validity was tested through source triangulation. Data analysis techniques included unitizing, sampling, recording, reducing, inferring, and narrating.
Results: In Textbook A, 5 misconceptions were found, consisting of 3 misidentifications, 1 overgeneralization, and 1 oversimplification. In Textbook B, there were 3 misconceptions, namely 1 overgeneralization and 2 oversimplifications.
Conclusion: There were misconceptions in the textbooks, both regarding the principles of genetic engineering and their applications.
References
Agrawal, S., & Rami, E. (2022). A Review: Agrobacterium-mediated Gene Transformation to Increase Plant Productivity. The Journal of Phytopharmacology, 11(2), 111–117. https://doi.org/10.31254/phyto.2022.11211
Aisyah, S., Noviyanti, E., & Triyanto. (2020). Bahan Ajar sebagai Bagian dalam Kajian Problematika Pembelajaran Bahasa Indonesia. Jurnal Salaka, 2(1), 62–65.
Aljabali, A. A. A., El-Tanani, M., & Tambuwala, M. M. (2024). Principles of CRISPR-Cas9 technology: Advancements in genome editing and emerging trends in drug delivery. In Journal of Drug Delivery Science and Technology (Vol. 92). Editions de Sante. https://doi.org/10.1016/j.jddst.2024.105338
Aly, A. A. & Borik, Z. M. (2023). Food crops, growth and productivity as an important focus for sustainable agriculture. In N. Guleria, P., Kumar, V., & Mo, B (Ed.), Plant Small RNA in Food Crops. Academic Press. https://doi.org/10.1016/B978-0-323-91722-3.00002-6
Ary, D., Jacobs, L. C., Sorensen, C., & Razavieh, A. (2015). Introduction to Research in Education (8th ed.). Cengange Learning.
Aslina, N., Azhari, M. I., Addieningrum, F. M., Mulyanto, & Sholeh, A. H. (2023). Analisis Kloning Dan Rekayasa Genetika Menurut Hukum Islam. Jurnal Addayyan, XVIII (II), 25-38. http://jurnalstaiibnusina.ac.id/index.php/AD
Atala, M. L. (2020). A Review Article: Bacterial Plasmids. Novateur Publications, 6(12), 8–12.
Bélanger, J. G., Copley, T. R., Hoyos-Villegas, V., Charron, J. B., & O’Donoughue, L. (2024). A comprehensive review of in planta stable transformation strategies. In Plant Methods (Vol. 20, Issue 1). BioMed Central Ltd. https://doi.org/10.1186/s13007-024-01200-8
Bhat, T.A. & Al-Khayri, J.M. (Eds.). (2023). Genetic Engineering Volume 1: Principles Mechanism, and Expression. Florida: Apple Academic Press.
Brown, T.A. (2025). Gene cloning & DNA analysis: an introduction, ninth edition. Manchester: John Wiley & Sons.
Cadacio, J. C. M. (2024). The Utilization of the Instructional Materials in Science 7 in the Public Secondary Schools in the First Congressional District, Division of Camarines Sur. IX(2454), 37–45. https://doi.org/10.51584/IJRIAS
Castaneda-barba, S., Top, E. M., & Stalder, T. (2025). Plasmids, a Molecular Cornerstone of Antimicrobial Resistance in the One Health Era. Nat Rev Microbiol, 22(1), 18–32. https://doi.org/10.1038/s41579-023-00926-x.Plasmids
Chen, C., Sonnert, G., Sadler, P. M., & Fredericks, C. (2020). The impact of student misconceptions on student persistence in a MOOC. J Res Sci Teach, 57, 879–910. https://doi.org/10.1002/tea.21616
Dale, J., James, A., Paul, J. Y., Khanna, H., Smith, M., Peraza-Echeverria, S., Garcia-Bastidas, F., Kema, G., Waterhouse, P., Mengersen, K., & Harding, R. (2017). Transgenic Cavendish bananas with resistance to Fusarium wilt tropical race 4. Nature Communications, 8(1). https://doi.org/10.1038/s41467-017-01670-6
Dale, J., Paul, J. Y., Dugdale, B., & Harding, R. (2017). Modifying Bananas: From Transgenics to Organics? Sustainability, 9(333), 1–13. https://doi.org/10.3390/su9030333
Damanik, C. A., Bayu, R., Simanullang, I. N. S., Sianipar, S. I., & Sihombing, W. L. (2025). Miskonsepsi Teorema Phytagoras pada Buku Ajar Siswa Sekolah Menegah Pertama dengan Perbandingan yang Benar. Pendas, 10(3), 250-254. https://doi.org/10.23969/jp.v10i03.33291
Debatisse, K., Brunie, M., Darracq, B., Bandini, E., Littner, E., Rocha, E. P. C., Mazel, D., & Loot, C. (2025). Bacterial natural transformation drives cassette shuffling and simplifies recombination in chromosomal integrons. Nucleic Acids Research, 53(21). https://doi.org/10.1093/nar/gkaf1162
Delafuente, J., & Diaz-colunga, J. (2024). Global epistasis in plasmid-mediated antimicrobial resistance. Mol Syst Biol, 20(4), 311–320. https://doi.org/10.1038/s44320-024-00012-1
Dewan, I., & Uecker, H. (2023). A mathematician’s guide to plasmids: an introduction to plasmid biology for modellers. Microbiology, 169(7), 1–23. https://doi.org/10.1099/mic.0.001362
Dikmenli, M., Çardak, O., & Öztaş, F. (2009). Conceptual Problems in Textbooks Conceptual Problems in Biology-Related Topics in Primary Science and Technology Textbooks in Turkey. International Journal of Enviromental & Science Education, 4(4), 429–440.
Domenech, A., Brochado, A. R., Sender, V., Hentrich, K., Henriques-Normark, B., Typas, A., & Veening, J. W. (2020). Proton Motive Force Disruptors Block Bacterial Competence and Horizontal Gene Transfer. Cell Host and Microbe, 27(4), 544-555.e3. https://doi.org/10.1016/j.chom.2020.02.002
Du, Y., Liu, Y., Hu, J., Peng, X., & Liu, Z. (2023). CRISPR/Cas9 systems: Delivery technologies and biomedical applications. Asian Journal of Pharmaceutical Sciences, 18(6), 1–31. https://doi.org/10.1016/j.ajps.2023.100854
Dubnau, D., & Blokesch, M. (2019). Mechanisms of DNA Uptake by Naturally Competent Bacteria. Annual Review of Genetics, 53, 217–237. https://doi.org/10.1146/annurev-genet-112618
Fakhriyah, F., Syukur, A., Aminah, S. K., Ratnasari, Y., & Roslan, R. (2025). Analysis of Misconceptions in the 2023 Revised Edition of 4th Grade Elementary School Science Textbook. JIPI (Jurnal IPA Dan Pembelajaran IPA), 9(1), 276–292.
Fan, L., Cheng, J., Xie, S., Luo, J., Wang, Y., & Sun, Y. (2021). Are textbooks facilitators or barriers for teachers’ teaching and instructional change? An investigation of secondary mathematics teachers in Shanghai, China. ZDM – Mathematics Education, 53(6), 1313–1330. https://doi.org/10.1007/s11858-021-01306-6
Fan, Y. (2024). Citation Fan Y (2024) Genetic Manipulation: Techniques and Applications. J Curr Neur Biol, 4, 20. https://doi.org/10.21767/JCNB-24.2.20
Fauziah, A., & Darvina, Y. (2019). Analisis Miskonsepsi Peserta Didik dalam Memahami Materi Gerak Lurus dan Gerak Parabola pada Kelas X SMAN 1 Padang. Pillar of Physics Education, 12(1), 73–80.
Gan, W. C., & Ling, A. P. K. (2022). CRISPR/Cas9 in plant biotechnology: applications and challenges. Biotechnologia, 103(1), 81–93. https://doi.org/10.5114/bta.2022.113919
Glick, B.R. & Patten, C.L. (2022). Molecular Biotechnology: Principles and Applications of Recombinant DNA Sixth Edition. Hokoben USA: John Wiley & Sons.
Goralogia, G. S., Willig, C., & Strauss, S. H. (2025). Engineering Agrobacterium for improved plant transformation. Plant Journal, 121(5), 1–16. https://doi.org/10.1111/tpj.70015
Guerra-Reyes, F., Guerra-Dávila, E., Naranjo-Toro, M., Basantes-Andrade, A., & Guevara-betancourt, S. (2024). Misconceptions in the Learning of Natural Sciences: A Systematic Review. Education Sciences, 14(497), 1–17.
Guleria, V. Kumar, & B. Mo. (Eds.), Plant Small RNA in Food Crops. India: Academic Press Elsevier.
Ham, A. Van Den, & Heinze, A. (2018). Studies in Educational Evaluation Does the textbook matter? Longitudinal effects of textbook choice on primary school students’ achievement in mathematics. Studies in Educational Evaluation, 59(July), 133–140. https://doi.org/10.1016/j.stueduc.2018.07.005
He, Y. (2022). Genetic Mapping usage in Better Diagnosis of Diseases. Advanced Techniques in Biology & Medicine, 10(11).
Helinski, D. R. (2022). A Brief History of Plasmids. EcoSal Plus, 10(1). https://doi.org/10.1128/ecosalplus.esp-0028-2021
Hull, S.C. (2025). Trait. Retrieved from https://www.genome.gov/genetics-glossary/Trait
Hwang, H.-H., Yu, M., & Lai, E.-M. (2017). Agrobacterium -Mediated Plant Transformation: Biology and Applications. The Arabidopsis Book, 15, e0186. https://doi.org/10.1199/tab.0186
Irawan, A. (2025). Basic Concepts and Importance of Educational Materials Development for Teachers and Students: General Theoretical Ideas. Al-Ahnaf: Journal of Islamic Education, Learning and …, 2(1), 167–177.
Jena, A. K., Pradhan, S., & Pradhan, P. (2024). Recombinant DNA Technology & Its Importance in Agriculture. Futuristic Trends in Biotechnology, 3(17), 978–993.
Jiang, F., & Doudna, J. A. (2017). CRISPR–Cas9 Structures and Mechanisms. Annu. Rev. Biophys., 46, 505–529. https://doi.org/10.1146/annurev-biophys
Jin, M., Liu, L., Wang, D. N., Yang, D., Liu, W. L., Yin, J., Yang, Z. W., Wang, H. R., Qiu, Z. G., Shen, Z. Q., Shi, D. Y., Li, H. B., Guo, J. H., & Li, J. W. (2020). Chlorine disinfection promotes the exchange of antibiotic resistance genes across bacterial genera by natural transformation. ISME Journal, 14(7), 1847–1856. https://doi.org/10.1038/s41396-020-0656-9
Kaif, A. (2023). Transgenic Organisms: Exploring the Promise and Controversies of Genetic Engineering. International Research Journal of Biotechnology, 14(3), 1–4. https://doi.org/10.14303/2141-5153.2023.51
Krippendorff, K. (2019). Content Analysis: An Introduction to Its Methodology (Fourth). SAGE Publications, Inc.
Kuball, J. (2025). Advancements in Genome Mapping for Genetic Research. 14(1000312), 1000312. https://doi.org/10.35248/2329-6682.25.14.312
Lewis, J., & Kattmann, U. (2004). Traits, genes, particles and information: Re-visiting students’ understandings of genetics. International Journal of Science Education, 26(2), 195–206. https://doi.org/10.1080/0950069032000072782
Li, F., & Wang, L. (2024). A study on textbook use and its effects on students’ academic performance. Disciplinary and Interdisciplinary Science Education Research, 6(4), 1–20. https://doi.org/10.1186/s43031-023-00094-1
Li, T., Yang, Y., Qi, H., Cui, W., Zhang, L., Fu, X., He, X., Liu, M., Li, P. feng, & Yu, T. (2023). CRISPR/Cas9 therapeutics: progress and prospects. Signal Transduction and Targeted Therapy, 8(1), 1–23. https://doi.org/10.1038/s41392-023-01309-7
Liao, H., Wu, J., VanDusen, N. J., Li, Y., & Zheng, Y. (2024). CRISPR-Cas9-mediated homology-directed repair for precise gene editing. Molecular Therapy Nucleic Acids, 35(4), 1–20. https://doi.org/10.1016/j.omtn.2024.102344
Liu, P.P. (2025). Transgenic. Retrieved from https://www.genome.gov/genetics-glossary/Transgenic
Ma, R., Yu, Z., Cai, Q., Li, H., Dong, Y., Oksman-Caldentey, K. M., & Rischer, H. (2020). Agrobacterium-mediated genetic transformation of the medicinal plant veratrum dahuricum. Plants, 9(2). https://doi.org/10.3390/plants9020191
Ministry of Education, Culture, Research and Technology. (2023). Data Pokok Pendidikan. Retrieved from https://dapo.kemdikbud.go.id/
Mithans, M., & Grmek, M. I. (2020). The Use of Textbooks in The Teaching Learning Process (A. Lipovec, J. Batic, & E. Kranjec (eds.)). University Press.
Mulia, N. & Zulyusri. (2021). Meta-Analisis Miskonsepsi Buku Teks Biologi SMA. Biodik, 7(1), 102-111. https://doi.org/10.22437/bio.v7i01.12220
Moran, M. N., Jones, D. B., Jensen, S. A., Marcoli, R., & Jerry, D. R. (2024). Optimising commercial traits through gene editing in aquaculture: Strategies for accelerating genetic improvement. Reviews in Aquaculture, 16(3), 1127–1159. https://doi.org/10.1111/raq.12889
Niazian, M., Sadatnoori, S. A., Galuszka, P., & Mortazavian, S. M. M. (2017). Tissue culture-based Agrobacterium-mediated and in Planta transformation methods. Czech Journal of Genetics and Plant Breeding, 53(4), 133–143. https://doi.org/10.17221/177/2016-CJGPB
Niedbała, G., Niazian, M., & Sabbatini, P. (2021). Modeling Agrobacterium-Mediated Gene Transformation of Tobacco (Nicotiana tabacum)—A Model Plant for Gene Transformation Studies. Frontiers in Plant Science, 12. https://doi.org/10.3389/fpls.2021.695110
Niu, C., Wu, H., Wang, X., Hu, L., Han, Y., & Qiao, J. (2025). Molecular mechanisms and applications of natural transformation in bacteria. Frontiers in Microbiology, 16, 1–17. https://doi.org/10.3389/fmicb.2025.1578813
Nketsiah, J., & Motlhabane, A. (2025). Evaluating Chemistry Textbooks in Ghanaian Senior High Schools: A Focus on The Presentation of Qualitative Analysis and Perspectives of Teachers and Learners. UNESA Journal of Chemical Education, 14(1), 8–21.
Nojadeh, J. N., Eryılmaz, N. S. B., & Ergüder, B. İ. (2023). CRISPR/CAS9 GENOME EDITING FOR NEURODEGENERATIVE DISEASES. In EXCLI Journal (Vol. 22, pp. 567–582). Leibniz Research Centre for Working Environment and Human Factors. https://doi.org/10.17179/excli2023-6155
Permadani, S. P., Aini, J. D. N., & Thohir, M. A. (2022). Identifikasi Miskonsepsi Materi Upaya Pelestarian dan Keseimbangan Alam Kelas V SDN Bendogerit 1. Jurnal Pembelajaran, Bimbingan, Dan Pengelolaan Pendidikan, 2(10), 916–924. https://doi.org/10.17977/um065v2i102022p916-924
Pratiwi, S., Purbosari, P. P., Aji, O. R., & Putra, R. R. (2024). Analysis of Genetic Engineering Material Misconceptions in Biology Textbooks for Ninth-Grade in Yogyakarta. Jurnal Pendidikan Sains, 12(2).
Prinz, A., Kollmer, J., Flick, L., Renkl, A., & Eitel, A. (2022). Refuting student teachers’ misconceptions about multimedia learning. Instructional Science, 50(1), 89–110. https://doi.org/10.1007/s11251-021-09568-z
Purkait, S., & Yousuf, P. (2024). Recombinant DNA Technology and Its Applications. Futuristic Trends in Biotechnology, 3(5), 219–236.
Riggs, A. D. (2021). Making, Cloning, and the Expression of Human Insulin Genes in Bacteria: The Path to Humulin. Endocrine Reviews, 42(3), 374–380. https://doi.org/10.1210/endrev/bnaa029
Salmon, B. (2025). The Role of Chromosome Mapping in Detecting and Managing Genetic Conditions Early. Journal of Down Syndrome & Chromosome Abnormalities, 11(278). https://doi.org/10.35248/2472-1115.25.11.278
Singh, N.R. (2023). Introduction to genetics. British Columbia: Thompson Rivers University.
Smyth, C., Leigh, R. J., Delaney, S., Murphy, R. A., & Walsh, F. (2022). Shooting hoops: globetrotting plasmids spreading more than just antimicrobial resistance genes across One Health. Microb. Genom., 8(8). https://doi.org/10.1099/mgen.0.000858
Soeharto, S., & Csap, B. (2022). Exploring Indonesian student misconceptions in science concepts. Heliyon, 8(May), 1–10. https://doi.org/10.1016/j.heliyon.2022.e10720
Sutrimah, Winarni, R., Wardani, N. E., & Ngadino. (2019). Evaluating the Use of Modern Indonesian Literary History Textbook (Poetry in East Java) in Teaching Literary History Course. International Journal of Instruction, 12(3), 1–16.
Syahyani, I. (2018). Analisis Miskonsepsi Materi Buku Pelajaran Biologi Kelas XII untuk Sekolah Menengah Atas. Inovasi Pendidikan, 5(2), 72–78.
Thomson, G., Dickinson, L., & Jacob, Y. (2024). Genomic consequences associated with Agrobacterium-mediated transformation of plants. Plant Journal, 117(2), 342–363.
Tikkanen, A. (2025). Crop agriculture. Retrieved from https://www.britannica.com/topic/crop-agriculture
Tore, O., Maria, K., & Andreas, L. (2023). Teachers’ Error handling Practices Within and Across Lesson Phases in the Mathematics Classroom. International Journal of Science and Mathematics Education, 1289–1314. https://doi.org/10.1007/s10763-022-10294-2
Vaghari-Tabari, M., Hassanpour, P., Sadeghsoltani, F., Malakoti, F., Alemi, F., Qujeq, D., Asemi, Z., & Yousefi, B. (2022). CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer. Cellular and Molecular Biology Letters, 27(49), 1–29. https://doi.org/10.1186/s11658-022-00348-2
Vioti, A. (2022). Procedure Involved in Recombinant DNA Technology Advancements in Genetic Engineering. Advan Genet Eng, 11(2), 1000193–1000194. https://doi.org/10.35248/2169-0111.22.11:193
Wang, S. W., Gao, C., Zheng, Y. M., Yi, L., Lu, J. C., Huang, X. Y., Cai, J. Bin, Zhang, P. F., Cui, Y. H., & Ke, A. W. (2022). Current applications and future perspective of CRISPR/Cas9 gene editing in cancer. Molecular Cancer, 21(57), 1–27. https://doi.org/10.1186/s12943-022-01518-8
Wardani, F., Khairuddin, & Rasyidah. (2023). Analisis Isu Sosio-Saintifik pada Buku Teks Biologi SMA Kelas XII Materi Bioteknologi. Bioedukasi, 14(1), 38–48.
Winter, M., Buckling, A., Harms, K., Johnsen, P. J., & Vos, M. (2021). Antimicrobial resistance acquisition via natural transformation: context is everything. Current Opinion in Microbiology, 64, 133–138. https://doi.org/10.1016/j.mib.2021.09.009
Yang, P., Zhang, S., Hu, D., Li, X., Guo, Y., Guo, H., Zhang, L., & Ding, X. (2024). Research Progress on the Mechanism and Application of the Type I CRISPR-Cas System. International Journal of Molecular Sciences, 25(23), 1–24. https://doi.org/10.3390/ijms252312544
Yuliati, Y. (2017). Miskonsepsi Siswa pada Pembelajaran IPA serta Remidiasinya. Bio Educatio, 2(2), 50–58.
Zajkov, O., Gegovska-zajkova, S., & Mitrevski, B. (2016). Textbook-Caused Misconceptions, Inconsistencies, and Experimental Safety Risks of a Grade 8 Physics Textbook. International Journal of Science and Mathematics Education, 14(1). https://doi.org/10.1007/s10763-016-9715-0
Zhang, S., Wang, Y., Lu, J., Yu, Z., Song, H., Bond, P. L., & Guo, J. (2021). Chlorine disinfection facilitates natural transformation through ROS-mediated oxidative stress. ISME Journal, 15(10), 2969–2985. https://doi.org/10.1038/s41396-021-00980-4
Zhu, Y. (2022). Advances in CRISPR/Cas9. BioMed Research International, 2022, 1–13. https://doi.org/10.1155/2022/9978571
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Purwanti Pratiwi Purbosari, Bayu Setiawam, Arief Abdillah Nurusman

This work is licensed under a Creative Commons Attribution 4.0 International License.




